近日，来自杭州富阳第一东说念主民病院和杭州师范大学附庸萧山病院的学者在BIOCELL上发表了题为Glycyrrhizic acid alleviates lung injury in sepsis through SIRT1/HMGB1 pathway的酌量著述。本文通过经受脂多糖（LPS）指导的BEAS-2B东说念主肺上皮细胞和盲肠结扎穿刺（CLP）大鼠两种本质模子，模拟脓毒症情况。证明甘草酸（GA）大略通过特定通路对脓毒症指导的急性肺挫伤（ALI）细胞挫伤和炎症具有权贵的保护作用。标明GA可能是诊治败血症和关系炎症的潜在诊治战略。

酌量布景

脓毒症是一种由全身炎症响应引起的危及生命的疾病，其最严重的并发症之一是急性肺挫伤 (ALI)，当今尚无针对脓毒症关系 ALI 的特定戒备或诊治步调，找到灵验的 ALI 诊治步调关于擢升患者的生涯率和生活质料至关紧要。高迁徙率族卵白 1 (HMGB1) 是一种具有双重功能的卵白质，动作晚期炎症介质，不仅与脓毒症磋商，还与脓毒症导致的急性肺挫伤磋商。Sirtuin 1 (SIRT1) 是一种 NAD+ 依赖性组卵白去乙酰化酶，具有等闲的生理作用，包括转念细胞应激抵触、炎症和代谢。抗炎机制一直是GA酌量的热门之一。先前的酌量发现，甘草酸 (GA) 不错权贵改善脓毒症 ALI 大鼠的肺组织病理景色，改善肺功能，镌汰肺组织的炎症水平。基于以往酌量，作家假定GA通过激活SIRT1转录因子阻扰HMGB1抒发，从而放松脓毒症肺挫伤。

酌量亮点

作家们通过在体外和体内酌量GA对感染性ALI的诊治后果，考据了GA通过SIRT1对LPS指导的BEAS-2B细胞发达保护作用（见图一）。而Western踪影和Co-IP法检测和评估已毕表露，GA通过SIRT1-HMGB1对LPS指导的BEAS-2B细胞发达保护作用（见图二）。对CLP大鼠的相应想法检测和相比已毕则标明，GA通过SIRT1在CLP大鼠中发达保护作用（见图三）。通过考据这些关系机制，作家旨在阐发GA对毒性ALI的诊治后劲，为其临床诓骗提供表面基础。

FIGURE 1. GA exerted a protective effect through SIRT1 in LPS-induced BEAS-2B cells. (A) qRT-PCR was used to test the relative SIRT1 mRNA expression in each group of BEAS-2B cells, n = 3 in each group; (B) CCK8 was used to evaluate the cell viability of the in each group of BEAS-2B cells, n = 6 in each group; (C and D) FCM was used to testify the apoptosis rate in each group of BEAS-2B cells, and the graph was shown, n = 3 in each group; (E) ELISA kits were used to testify the expression of TNF-α, IL-6, IL-1β in each group of BEAS-2B cells, n = 6 in each group, ▲▲p < 0.01 vs. control group, **p < 0.01 vs. LPS group, ##p < 0.01 vs. GA + LPS + si-NC group.

Note: Glycyrrhizic acid: GA; SIRT1: Sirtuin 1; LPS: lipopolysaccharide; qRT-PCR: quantitative real time polymerase chain reaction; CCK8: Cell Counting Kit-8; FCM: Flow CytoMetry; ELISA: enzyme-linked immunosorbent assay; TNF-α: Tumor Necrosis Factor-α; IL-6: interleukin-6; IL-1β: interleukin-1β.

FIGURE 2. GA exerted a protective effect through SIRT1-HMGB1 in LPS-induced BEAS-2B cells. (A and B) Western blot was applied to test the relative expression of the SIRT1 and HMGB1 in each group of BEAS-2B cells, n = 3 in each group; (C and D) Co-IP was used to evaluate the inner relation of the SIRT1 and HMGB1 in each group of BEAS-2B cells, n = 3 in each group; ▲p < 0.05 and ▲▲p < 0.01 vs. control group, **p < 0.01 vs. LPS group, ##p < 0.01 vs. GA + LPS + si-NC group.

Note: Glycyrrhizic acid: GA; SIRT1: Sirtuin 1; LPS: lipopolysaccharide; HMBG1: high mobility group box 1; Co-IP: Co-Immunoprecipitation.

FIGURE 3. GA exerted a protective effect through SIRT1 in CLP rats. (A) Wet-dry ratio was used to detect the wet/dry weight ratio of lung tissue in rats, n = 6 in each group; (B and C) The representative image of the pathology in the lung tissue and HE score in rats were recorded, n = 3 in each group; (D–F) The contents of ALT, AST, SCr and BUN in peripheral blood of rats in each group were detected by automatic biochemical analyzer; and the kit was used to detect MPO activity of lung tissue in rats, n = 6 in each group; (G) The levels of IL-6, IL-1β and TNF-α in peripheral blood in rats were detected by ELISA, n = 6 in each group. ▲▲p < 0.01 vs. sham group, **p < 0.01 vs. CLP group, ##p < 0.01 vs. CLP + GA group.

Note: Glycyrrhizic acid: GA; SIRT1: Sirtuin 1; CLP: Cecum Ligation and Puncture; HE: hematoxylin and eosin; ALT: alanine transaminase; AST: aspartate transaminase; SCr: serum creatinine; BUN: blood urea nitrogen; MPO: myeloperoxidase; ELISA: enzyme-linked immunosorbent assay; TNF-α: Tumor Necrosis Factor-α; IL-6: interleukin-6; IL-1β: interleukin-1β.

筹计议断

脓毒症中细胞挫伤、炎症和潜在分子通路之间的复杂关系一直是浩荡酌量的焦点。本酌量已毕揭示了 SIRT1-HMGB1 通路在介导 GA 在 LPS 指导的 BEAS-2B 细胞和 CLP 大鼠中的作用中的关键作用，为脓毒症和关系疾病提供了潜在的诊治靶点（见图四）。本酌量中的卵白质踪影和 Co-IP 分析揭示了 SIRT1 和 HMGB1 抒发之间的反比关系，这标明GA可能通过转念 SIRT1-HMGB1 轴发达其保护作用。同期，本酌量探索了GA 通过转念 SIRT1-HMGB1 通路对脓毒症诱发的急性肺挫伤的保护作用。使用 LPS 指导的东说念主肺上皮细胞和 CLP 大鼠模子，作家证明 GA 通过擢升 SIRT1 水和睦镌汰 HMGB1 抒发来减少肺组织挫伤、炎症和细胞凋一火。这些已毕强调了GA大略通过 SIRT-HMGB1 通路起作用从而具备诊治脓毒症引起的细胞挫伤和炎症的诊治剂的后劲。

FIGURE 4. GA exerted a protective effect through SIRT1-HMGB1 in CLP rats. (A and B) TUNEL staining was used to evaluate the percentage of the apoptosis cell in lung tissue in rats (magnification 200 ×, scale bar: 100 μm), n = 3 in each group; (C and D) Western blot was applied to test the relative expression of the SIRT1 and HMGB1 in rats, n = 3 in each group; ▲▲p < 0.01 vs. sham group, **p < 0.01 vs. CLP group, #p <0.05 and ##p < 0.01 vs. CLP + GA group.

Note: Glycyrrhizic acid: GA; SIRT1: Sirtuin 1; CLP: Cecum Ligation and Puncture; HMBG1: high mobility group box 1.

援用样子

APA Style

LIN, B., YING, X., ZHANG, C., ZHANG, G. (2024). Glycyrrhizic acid alleviates lung injury in sepsis through SIRT1/HMGB1 pathway. BIOCELL, 48(11), 1613-1623. https://doi.org/10.32604/biocell.2024.053652

Vancouver Style

LIN B, YING X, ZHANG C, ZHANG G. Glycyrrhizic acid alleviates lung injury in sepsis through SIRT1/HMGB1 pathway. BIOCELL . 2024;48(11):1613-1623 https://doi.org/10.32604/biocell.2024.053652

IEEE Style

B. LIN, X. YING, C. ZHANG, and G. ZHANG, “Glycyrrhizic acid alleviates lung injury in sepsis through SIRT1/HMGB1 pathway,” BIOCELL , vol. 48, no. 11, pp. 1613-1623, 2024. https://doi.org/10.32604/biocell.2024.053652

BIOCELL期刊简介

主编

Alfredo Castro-Vazquez

Manuel A. Barbieri

BIOCELL 是一册开源月刊，旨在发表分子细胞生物学领域的高质料论文。

本刊关怀的主题包括但不限于：细胞生物学、结构与分子生物学、免疫学、病理学和神经生物学、基因组学、转录组学、卵白质组学和代谢组学、植物分子与细胞生物学、筹算生物学等。

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